Find and download the sequence of Pseudechis australis basic phospholipase A2-PA5 from Uniprot. Use ...

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Find and download the sequence of Pseudechis australis basic phospholipase A2-PA5 from Uniprot. Use Blast (Entrez or Uniprot) to find a diverse set of orthologs. None should be more than N80 % sequence identity, but be sure to include examples down to 50 % sequence identity (without other data, it can be difficult to be sure whether the proteins are orthologs below this sequence identity level); make sure no two pairs of sequences exceed the 80% sequence identity cutoff. Pay attention to the N-and Ctermini of selected sequences, and exclude any where you suspect sequencing or annotation errors. Search paperblast to find at least one reasonable homolog with a concrete analysis of potential functions) preferably a robust demonstration of a function. Search against the pdb to find the closest homolog for which there is a structure. Make sure you have the genomic version of the pdb sequence, not the cloned version (which will likely contain a His tag and other artifacts; use the Uniprot link on the pdb site). Make a multiple sequence alignment, a phylogenetic tree (MABL), and a web logo. The MSA should include 10 -15 sequences, the phylogenetic tree 20-30 (include all of your MSA sequences), the Weblogo can be built with the phylogenetic set. Weblogo can get difficult to read if the sequence is too long. Submitting the alignment in chunks of 50 — 100 a.a. can help here. Make sure that all sequences are complete, that sequences are labeled by species of origin, and that the images you create are publication quality (espript). Combine all three panels into one figure, and submit as a multi-panel figure with a caption; make sure to identify the sequence(s) with structure and published papers. Your caption should give the corresponding UNIPROT or accession.version numbers of all the sequences that appear in the figure.